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Title:
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Authors:
Betzig, Eric; Patterson, George H.; Sougrat, Rachid; Lindwasser, O. Wolf; Olenych, Scott; Bonifacino, Juan S.; Davidson, Michael W.; Lippincott-Schwartz, Jennifer; Hess, Harald F.
Affiliation:
AA(Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA.; New Millennium Research, LLC, Okemos, MI 48864, USA.), AB(Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development (NICHD), Bethesda, MD 20892, USA.), AC(Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development (NICHD), Bethesda, MD 20892, USA.), AD(Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development (NICHD), Bethesda, MD 20892, USA.), AE(National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310, USA.), AF(Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development (NICHD), Bethesda, MD 20892, USA.), AG(National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310, USA.), AH(Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development (NICHD), Bethesda, MD 20892, USA.), AI(NuQuest Research, LLC, La Jolla, CA 92037, USA.)
Publication:
Science, Volume 313, Issue 5793, pp. 1642-1645 (2006).
Publication Date:
09/2006
Category:
BIOCHEM
Origin:
SCIENCE
Abstract Copyright:
(c) 2006: Science
DOI:
10.1126/science.1127344
Bibliographic Code:
2006Sci...313.1642B

Abstract

We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ~2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method-termed photoactivated localization microscopy-to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.
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