Sign on

SAO/NASA ADS General Science Abstract Service

· Find Similar Abstracts (with default settings below)
· Electronic Refereed Journal Article (HTML)
· Full Refereed Journal Article (PDF/Postscript)
· Reads History
·
· Translate This Page
Title:
Synthesis of fluorescent nucleotide analogues: 5'-mono-, di-, and triphosphates of linear-benzoguanosine, linear-benzoinosine, and linear-benzoxanthosine.
Authors:
Leonard, Nelson J.; Keyser, Gene E.
Publication:
Proceedings of the National Academy of Sciences of the United States of America, Volume 76, Issue 9, 1979, pp.4262-4264
Publication Date:
09/1979
Origin:
JSTOR; PNAS
DOI:
10.1073/pnas.76.9.4262
Bibliographic Code:
1979PNAS...76.4262L

Abstract

The fluorescent nucleotide analogues (the 5'-mono-, di-, and triphosphates of lin-benzoguanosine, lin-benzoxanthosine, and lin-benzoinosine) have been prepared for use as dimensional probes of enzyme binding sites. They have quantum yields in aqueous solution of 0.39, 0.55, and 0.04 and fluorescent lifetimes of 6, 9, and approximately equal to 1.5 nsec, respectively. lin-Benzoinosine 5'-monophosphate is a substrate for xanthine oxidase (xanthine:oxygen oxidoreductase, EC 1.2.3.2), providing lin-benzoxanthosine 5'-monophosphate, and lin-benzoinosine 5'-diphosphate is a substrate for polynucleotide phosphorylase (polyribonucleotide:orthophosphate nucleotidyltransferase. EC 2.7.7.8), giving poly(lin-benzoinosinic acid). The benzologues of the purine diphosphates are substrates for pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40), which is used to prepare the triphosphates.
Bibtex entry for this abstract   Preferred format for this abstract (see Preferences)

Find Similar Abstracts:

Use: Authors
Title
Abstract Text
Return: Query Results Return    items starting with number
Query Form
Database: Astronomy
Physics
arXiv e-prints